ZEISS Correlative Cryo Workflow is a combined hardware and software solution designed to streamline correlative cryogenic microscopy. It connects widefield, confocal (LSM), and focused ion beam scanning electron microscopes (FIB-SEM) in a seamless workflow, enabling researchers to localize fluorescent macromolecules, perform high-contrast volume imaging, and efficiently prepare TEM lamellae under cryogenic conditions. The workflow includes dedicated accessories for safe sample transfer, cryo-compatible objectives, and high-sensitivity detectors to maintain sample integrity and data quality. Data management is handled by ZEN Connect, which keeps all imaging data in context throughout the workflow. The system is optimized for life science research, particularly for studying cellular ultrastructure in a near-to-native state, and supports both cryogenic and room temperature applications.

Key Features

Correlative Cryogenic Workflow Connects multiple microscopy modalities for comprehensive analysis.

• Integrates widefield, confocal (LSM), and FIB-SEM microscopes.
• Enables seamless transfer and correlation of data between modalities.

High-Resolution Imaging Under Cryo Conditions Preserves sample integrity and ultrastructure.

• Cryo-compatible objectives and Airyscan detector for high sensitivity.
• Maintains low temperatures to prevent devitrification.

Efficient TEM Lamella Preparation Streamlines sample preparation for electron tomography.

• On-grid lamella thinning with FIB-SEM.
• Dedicated accessories for safe cryo sample handling.

Integrated Data Management Keeps all imaging data organized and in context.

• ZEN Connect software for correlative microscopy data management.
• Processing tools for image enhancement and workflow documentation.

Flexible Application Supports both cryogenic and room temperature workflows.

• Microscopes can be used for a wide range of applications beyond cryo.

Benefits

Comprehensive Structural and Functional Insights Combines multiple imaging modalities for a thorough understanding of samples.

• Correlates fluorescence and electron microscopy data for multi-scale analysis.
• Enables study of cellular ultrastructure in near-native state.

Streamlined, Reproducible Workflow Reduces complexity and risk in cryogenic sample handling.

• Dedicated accessories and software simplify the workflow.
• Ensures safe transfer and consistent results.

Maximized Instrument Utilization Supports both cryogenic and standard imaging.

• Instruments can be used for room temperature applications when not needed for cryo.

Enhanced Data Management and Collaboration Centralizes and contextualizes all imaging data.

• ZEN Connect enables efficient data tracking and sharing.