ZEISS ZEN Connect Toolkit is an advanced software module within the ZEISS ZEN microscopy suite, designed to facilitate correlative and multi-modal imaging workflows. The toolkit provides comprehensive tools for acquiring, aligning, and registering images from different microscopy modalities, such as light and electron microscopy, within a unified project environment. It enables users to intuitively align images, perform auto-registration using calibrated stages, and define or retrieve regions of interest across multiple systems. The toolkit supports advanced features like 3D alignment (with the 3D Toolkit), measurement functions in the correlative viewer, and extended image or movie export. It is particularly suited for research environments where combining data from various imaging techniques is essential for comprehensive sample analysis. The Connect Toolkit extends the basic correlative functions available in the standard ZEN installation, offering a full suite of tools for complex, reproducible, and efficient correlative microscopy workflows.

Key Features

Multi-Modal Image Acquisition and Registration Enables integration and alignment of images from different microscopy systems.

• Acquire and import images from various modalities (e.g., light, electron microscopy).
• Auto-registration with calibrated microscope stages for precise overlay.
• Correlative viewer for unified project management.

Advanced Alignment and Measurement Tools Provides intuitive and automated tools for image alignment and analysis.

• Alignment wizards and 3D (z-dimension) alignment (with 3D Toolkit).
• Measurement functions directly in the correlative viewer.
• Define and retrieve regions of interest across systems.

Workflow and Export Capabilities Streamlines complex imaging workflows and data sharing.

• Stage control from the viewer for seamless navigation.
• Extended image and movie export, including project views and SerialEM support.
• Import of Bioformats and FIB stack images.

Benefits

Comprehensive Correlative Imaging Facilitates integration of data from multiple imaging techniques.

• Enables holistic sample analysis by combining complementary data.
• Reduces manual effort and potential for misalignment.

Reproducible and Efficient Workflows Standardizes and automates complex imaging processes.

• Ensures consistent results across experiments and users.
• Saves time in multi-step, multi-system imaging workflows.

Enhanced Data Management and Sharing Supports collaborative and transparent research.

• Unified project environment for all correlative data.
• Easy export and sharing of results for documentation and publication.