Visium HD Spatial Gene Expression is a cutting-edge product by 10x Genomics that enables whole transcriptome gene expression analysis at single-cell scale with continuous tissue coverage. This innovative technology allows researchers to perform precise transcript localization and generate high-quality spatial transcriptomics data from various sample types, including human and mouse tissues

Key Features Next-generation slide architecture Visium HD slides contain two 6.5 x 6.5 mm Capture Areas with millions of 2 x 2 µm barcoded squares, achieving single cell-scale spatial resolution

• .Continuous lawn of oligonucleotides without gaps
• Data output at 2 µm and multiple bin sizes

Versatile sample compatibility The technology works with diverse tissue types and preparation methods

• .Compatible with fresh frozen, fixed frozen, and FFPE samples
• Suitable for archived blocks and pre-sectioned slides

Visium CytAssist integration Enhances spatial data quality with a simplified workflow

Powerful visualization tools Seamlessly combines histological and gene expression data

• .Map gene expression onto H&E or IF images
• Identify gene signatures of neighboring regions

Benefits High-resolution spatial transcriptomics Provides unparalleled insights into tissue architecture and cellular function

• .Single cell-scale resolution
• Continuous tissue coverage

Flexible sample processing Accommodates various tissue preparation methods and types

• .Works with FFPE, fresh frozen, and fixed frozen tissues
• No optimization required for different sample types

Comprehensive data analysis Offers powerful software tools for in-depth exploration of spatial gene expression

• .Space Ranger for automatic analysis and cluster identification
• Loupe Browser for interactive visualization of results