Powering Discovery with AI

Quantitative and intuitive image analysis for pathology

Image Analysis Simplified

With unmatched ease-of-use and scalability, powerful analytic capabilities, and the fastest processing speeds available for digital pathology, research, pharma and healthcare organizations worldwide are using HALO® for high-throughput, quantitative tissue analysis.

Intuitive Platform for Analysts at All Levels​

An image analyst can be up and running with basic analysis functions in just a couple of hours with the user-friendly HALO platform – even with no prior image analysis experience! ​ HALO modules simplify the analysis workflow so there is no need to ‘build’ algorithms from scratch. Our purpose-built modules make HALO easy-to-use and understand which means you can spend less time learning software and more time analyzing data.​

Extensive Analytics, Reliable Results​

HALO reports morphological and multiplexed expression data on a cell-by-cell basis across entire tissue sections while maintaining an interactive link between cell data and image. Sort and filter cell data to mine millions of cells while also visually assessing cell populations in the context of tissue architecture, leverage interactive markups to understand colocalization and explore combined phenotypes, and define custom analysis outputs.

Leverage Integrated AI Tools

Use pre-trained deep-learning networks for optimized nuclear and membrane segmentation directly in the HALO platform with cell analysis modules in brightfield or fluorescence, including the Multiplex IHC and Highplex FL modules.

Powering Discovery with AI: Features of the HALO Image Analysis Platform

View Settings

Optimize fluorescent image visualization using powerful view settings capabilities. Compare channel histograms of multiple images, track intensity values in the histogram per channel, leverage an autofit calculation, and apply settings in batch.

Annotation Tools​

Annotate and explore images with a variety of freehand tools, reticle-based tools, toggle markup images, explore intensity data, measure distances, or take a quick snapshot.​

AI Powered Nuclear and Membrane Segmentation

With pre-trained AI-based networks for nuclear and membrane segmentation available in both brightfield and fluorescence, optimizing your segmentation parameters has never been easier.

Real-Time Tuning

The powerful real-time tuning feature of HALO provides live feedback on image analysis parameters.

​Phenotype Setup​

Identify cell types of interest with the streamlined phenotype editor feature. Assign channel combinations using an intuitive grid interface, visualize phenotypes using the real-time tuning feature, and export phenotype settings as .csv files. ​

Customized Analysis Outputs

Customize image analysis outputs and set up calculations prior to running analysis to create exactly the data you need.

Interactive Markups

Investigate results with interactive markup images where you can toggle on and off each cell population of interest.

Cell-by-Cell Data​

Navigate back and forth between the cell-by-cell data and the image markup with ease to explore cell populations of interest in the context of the tissue architecture. Sort cells according to biomarker intensity and immediately locate cells with highest intensity in the image, examine particular phenotypes of interest, and more.​

Heatmaps

​Gain spatial insights with the built-in heat map function of HALO. Explore the density of immune cells surrounding a tumor or investigate the density of any other population of interest.

Batch Analysis

Use saved analysis settings to queue up a batch analysis in just a few clicks. Continue to work in HALO while the automated analysis runs on the back end.​

​Image Registration

Register serial IHC-stained sections in HALO and synchronize navigation to explore image analysis results. Interested in developing a tissue classifier on one image and applying it on the rest? Learn more about our Serial Section Analysis Add-on tool.

​Figure Maker

Acquire publication quality figures in just a few clicks with the figure maker feature. Quickly define the number of frames, select images of interest from across studies, then optimize reference frames, scale bars, labels, and more. Save figures to edit later or download at your desired image size and resolution.​​​

Advanced Data Export​

With the advanced export feature of HALO, you can customize an export to provide exactly the data you need for downstream analysis. Options include exporting annotations, analysis or classifier settings, reports, summary data, object data in a spreadsheet or FCS format, and more.​