10x Genomics' CytAssist Spatial Gene and Protein Expression is an advanced spatial biology solution that enables researchers to simultaneously analyze whole transcriptome gene expression and highly multiplexed protein expression from a single tissue section with spatial resolution. This innovative technology provides a comprehensive view of tissue organization by combining protein detection, mRNA expression, and morphological data.

Key Features

Multiomics Analysis Enables simultaneous study of protein and RNA from a single tissue section

• Uses a curated, pre-validated antibody content panel
• Provides whole transcriptome detection

Comprehensive Tissue Profiling Allows visualization of protein detection and gene expression across entire tissue sections

• No need to select regions of interest
• Unbiased gene expression analysis

Morphology Integration Unique workflow that preserves tissue morphology

• Allows H&E or IF staining before other chemical treatments
• Combines histological analysis with molecular profiling

Data Analysis and Visualization Streamlined analysis of complex spatial data

• Includes Space Ranger analysis software for automatic data processing
• Features Loupe Browser for interactive data exploration

Benefits

Enhanced Scientific Insights Provides a deeper understanding of biological complexity

• Enables discovery of new biomarkers and cell types
• Facilitates mapping of spatial organization in cell atlases

Improved Experimental Efficiency Maximizes data output from limited tissue samples

• Generates multiple data types from a single tissue section
• Eliminates need for separate protein and RNA analyses

Overcoming Technical Limitations Addresses challenges in traditional protein detection methods

• Avoids spectral overlap issues in fluorescence-based methods
• Allows for higher multiplexing of protein targets

Versatile Sample Compatibility Designed for use with various tissue types

• Compatible with FFPE (formalin-fixed, paraffin-embedded) samples
• Supports analysis of human immune cell-containing tissues